Abundance and Compositions of B-Vitamin-Producing Microbes in the Mammalian Gut Vary Based on Feeding Strategies

Abstract

Microbial communities offer numerous physiological services to their hosts, but we still have a poor understanding of how these functions are structured across mammalian species. Specifically, our understanding of processes of vitamin synthesis across animals is severely limited. Mammals maintain close associations with gut microbes that provide numerous nutritional benefits, including vitamin synthesis. While most mammals obtain sufficient vitamins from their diets, deficiencies in various B vitamins (biotin, cobalamin, riboflavin, thiamine, etc.) are reported in captive animals. Biomedical and agricultural research has shown that gut microbes are capable of synthesizing B vitamins and assisting with host vitamin homeostasis. However, we have a poor understanding of distribution and abundance of B-vitamin synthesis across mammalian hosts. Here, we leveraged a publicly available metagenomic data set from 39 mammalian species and used MG-RAST to compare the abundance and composition of B-vitamin-synthesizing microbes across mammalian feeding strategies. We predicted that herbivores would have the highest abundance of genes associated with vitamin synthesis, as plant material is often low in B vitamins. However, this hypothesis was not supported. Instead, we found that relative abundances of genes associated with cobalamin and thiamine synthesis were significantly enriched in carnivorous mammals. The taxonomic community structure of microbes predicted to be involved in B-vitamin synthesis also varied significantly based on host feeding strategy. For example, the genus Acinetobacter primarily contributed to predicted biotin synthesis in carnivores but was not predicted to contribute to biotin synthesis in herbivores or omnivores. Given that B vitamins cannot be stored within the body, we hypothesize that microbial synthesis of B vitamins could be important for wild carnivores that regularly experience periods of fasting. Overall, these results shed light on the distribution and abundance of microbial B-vitamin synthesis across mammalian groups, with potential implications for captive animals. IMPORTANCE Microbial communities offer numerous physiological services to their hosts, but we still have a poor understanding of how these functions are structured across mammalian species. Specifically, our understanding of processes of vitamin synthesis across animals is severely limited. Here, we compared the abundance of genes associated with the synthesis of B vitamins and the taxonomic composition of the microbes containing these genes. We found that herbivores, omnivores, and carnivores harbor distinct communities of microbes that putatively conduct vitamin synthesis. Additionally, carnivores exhibited the highest abundance of genes associated with synthesis of specific B vitamins, cobalamin and thiamine. These data uncover the potential importance of microbes in the vitamin homeostasis of various mammals, especially carnivorous mammals. These findings have implications for understanding the microbial interactions that contribute to the nutritional requirements of animals held in captivity.