Comparison of Enzyme-Immunoassay and Rapid Immunochromatography Test for Detecting Helicobacter pylori Stool Antigen

Abstract

The rapid immunochromatography for Helicobacter pylori stool antigen (HpSA) test is a recently developed method for detection of Heicobacter pylori (H. pylori) infection. The objective of this study was to compare rapid HpSA test with a conventional enzyme-immunoassay (EIA) and to determine the prevalence of H. pylori infection in symptomatic (recurrent abdominal pain/RAP) Indonesian children. One hundred and two high school children aged 12-18 years old in Bandung with RAP from July to November 2008 were enrolled, excluding children who had additional symptoms, such as diarrhea or fever. First study was performed in 102 samples in laboratory of Leiden University Medical Center (LUMC), Helicobacter pylori infection was detected by a conventional EIA and rapid test. A second study was performed on a stored collection of 32 feces samples previously tested positive for H. pylori by EIA in laboratory of LUMC; these samples were retested by both assays. The results showed the sensitivity, specificity, positive predictive value, and negative predictive value of the rapid test were 100%, 98.2%, accuracy 98.5%, 92.6% and 100%, respectively, from 134 tested samples. The overall prevalence of H. pylori infection among Indonesian children was 3% by EIA at first testing. In conclusion, the prevalence of H. pylori infection among symptomatic Indonesian children is very low and the new developed Pylori-Strip test shows good performance, very fast, and easy to use compared to EIA. [MKB. 2014;46(1):52-6]