Incorporation of Oxygen into Glycolate, Glycine, and Serine during Photorespiration in Maize Leaves

Abstract

Glycolate, glycine, and serine extracted from excised Zea mays L. leaves which had been allowed to photosynthesize in the presence of (18)O(2) were analyzed by gas chromatography-mass spectrometry. In each case, only one of the oxygen atoms of the carboxyl group had become labeled. The maximum enrichment observed in glycine and serine was attained after 5 minutes and 15 minutes of exposure to (18)O(2) at the CO(2) compensation point; the labeling was very high, reaching 70 to 73% of that in the applied O(2). Thus, it appears that all or nearly all of the glycine and serine are synthesized in maize leaves via fixation of O(2). In the presence of CO(2) (380 or 800 microliters per liter), (18)O-labeling was markedly slower.Glycolate enrichment was variable and much lower than that in glycine and serine. It is possible that there are additional pathways of glycolate synthesis which do not result in the incorporation of (18)O from molecular oxygen. An estimation of the metabolic flow of O(2) through the photorespiratory cycle was made. It appeared that less than 75% of the O(2) taken up by maize leaves is involved in this pathway. Therefore, other processes of O(2) metabolism must occur in the light.