Genetic plasticity among genotypes of Rosa centifolia and R. damascena from Pakistan, USA and Iran

Abstract

Molecular analysis of various genotypes is the key component for evaluating their true to type genotype to start a commercial business and for future breeding projects. This study was performed to investigate the genetic diversity among genotypes of Rosa centifolia and R. damascena from Pakistan, USA and Iran. Thirty-six genotype of rose were analyzed with thirteen microsatellite markers scattered throughout the genome. The exclusive analysis of twenty-four R. Centifolia genotypes from Pakistan by UPGMA and PCA demonstrated genetic divergence among the districts, while high level of homology within the district. On the other hand, comparison of Pakistani genotypes with USA and Iran, R. centifolia Faisalabad and R. centifolia Sargodha, were found genetically divergent to Fantin-Latour and Paul Ricault but closely related with cabbage rose 2. R. damascena Faisalabad was found genetically diverse from other genotypes of R. damascena from Pakistan and Iran, while genetically closer to Rose de Meaux, Gros Choux d'Hollande and Fantin Latour of R. centifolia genotypes from USA. Genetic diversity of R. centifolia for Pakistani genotypes showed highly conserved nature at district level. In comparison, Pakistani genotypes of R. centifolia have maximum genetic diversity from USA genotypes except for Cabbage rose2 which showed their common ancestor. Findings of this study will help to construct a database helpful for characterization of other rose genotypes having economic importance and can be utilized for marker assisted breeding for improvement of R. centifolia.