A simultaneous determination of honokiol and mangolol in oriental pharmaceutical decoctions containing Magnolia bark by ion-pair high-performance liquid chromatography. II

Abstract

A simple method using ion-pair high-performance liquid chromatography was established for the rapid and precise determination of honokiol(3',5-di-2-propenyl-1,1'-biphenyl-2,4'-diol) and magnolol(5,5'-di-2-propenyl-1,1'-biphenyl-2,2'-diol) in eighteen species of oriental pharmaceutical decoctions containing Magnolia bark. An ODS column and a mixed solvent system of water involving 10 mM tetra-n-amylammonium bromide (TAA) and acetonitorile (4:6) as a mobil phase were used for the separation. Honokiol and magnolol were eluted without interference of other coexisting components within 12 min.