The First Serological Detection and Risk Factors Analysis of Akabane Virus in Egyptian Cattle

Abstract

Akabane virus (AKAV) is an insect-borne virus belonging to the genus Orthobunyavirus of the family Peribunyaviridae. It is the etiologic agent of Akabane disease (AD), which emerged in Asia, Australia, and the Middle East causing severe economic losses among domestic and wild animals. AKAV has not received enough attention in Egypt, and its evidence among Egyptian animals has never been reported. Therefore, this study used ELISA assay to investigate the seroprevalence of AKAV among Egyptian dairy and beef cattle in eight localities of Beheira province, north Egypt. Out of 368 investigated plasma samples, the overall AKAV seroprevalence was 54.3% (95% CI: 50.8–61.4). AKAV antibodies were detected in all examined cattle farms (7/7) and the majority of abattoirs (8/9). Age, sex, breed, and location of the tested cattle were analyzed as risk factors for AKAV infection. A higher significant increase in seropositivity was obtained in cattle who were aged >5 years (p < 0.0001; OR = 9.4), females (p < 0.0001, OR = 8.3), or Holstein breed (p < 0.0001, OR = 22.6) than in younger ages, males, and Mixed and Colombian zebu breeds, respectively. Moreover, a significant variation in AKAV seroprevalence between the tested locations was noticed. Ultimately, a multivariable analysis concluded that age (p = 0.002, OR = 3.32, 95% CI = 1.57–7.04) and breed (p = 0.03, OR = 1.69, 95% CI = 1.05–2.72) were significant risks for AKAV infection. In conclusion, this study is the first to detect AKAV infection in Egyptian animals.