Isolation and production of proteases from bacteria isolate from Bekasam and Rusip (Traditional Food from South Sumatera, Indonesia)

Abstract

The research aims to assay enzyme activity of bacteria from traditional fermentation product (bekasam and rusip). The bacteria were inoculated in skim milk agar (SMA), and will produce clear zone. The best isolates from 9 isolates of bekasam and 9 isolates from rusip will be choosen, and the best result from bekasam and rusip were A3K2 and B3K2, respectively. Index proteolitic of A3K2 and B3K2 isolates were 10.0 and 7.0, respectively. Optimum time production of protease from A3K2 and B3K2 were 16 h and 32 h, respectively.