Highly effcient system for plant regeneration from leaf and stem explants in Dahlia

Abstract

Protocol of plant regeneration from leaf and stem explants was established in dahlia (Dahlia×pinnata) 'Yamatohime' in in vitro culture. Nodular calli were successfully induced from leaf and stem explants on 30 g l-1 sucrose-containing MS medium supplemented singly with one of the cytokinins, 6-benzyladenine (BA), zeatin or thidiazuron (TDZ) and the highest frequency of nodular callus induction was obtained on medium containing 10 mg l-1 TDZ. Te nodular callus induced on this medium subsequently produced a tissue with numerous shoot primordial structures called 'mass of shoot primordia' (MSP), which was maintained at least for more than 2 years on the same medium. Shoot induction occurred afer transfer the MSP onto hormone-free MS medium, in which replacement of sucrose by maltose was necessary. Te shoots excised from the MSP were rooted on hormone-free MS medium and successfully grown under greenhouse conditions. Te regenerated plants showed the same morphological characteristics to those of the control plants and fowered normally. copy; 2013 The Japanese Society for Plant Cell and Molecular Biology.