Normal fertility requires the expression of carbonic anhydrases II and IV in sperm

Abstract

HCO3- is a key factor in the regulation of sperm motility. High concentrations of HCO3- in the female genital tract induce an increase in sperm beat frequency, which speeds progress of the sperm through the female reproductive tract. Carbonic anhydrases (CA), which catalyze the reversible hydration of CO2 to HCO3-, represent potential candidates in the regulation of the HCO3- homeostasis in sperm and the composition of the male and female genital tract fluids. We show that two CA isoforms, CAII and CAIV, are distributed along the epididymal epithelium and appear with the onset of puberty. Expression analyses reveal an up-regulation of CAII and CAIV in the different epididymal sections of the knockout lines. In sperm, we find that CAII is located in the principal piece, whereasCAIVis present in the plasma membrane of the entire sperm tail. CAII and CAIV single knockout animals display an imbalanced HCO3- homeostasis, resulting in substantially reduced sperm motility, swimming speed, and HCO3--enhanced beat frequency. The CA activity remaining in the sperm of CAII-and CAIV-null mutants is 35% and 68% of that found in WT mice. Sperm of the double knockout mutant mice show responses to stimulus by HCO3- or CO2 that were delayed in onset and reduced in magnitude. In comparison with sperm from CAII and CAIV double knockout animals, pharmacological loss of CAIV in sperm from CAII knockout animals, show an even lower response to HCO3-. These results suggest that CAII and CAIV are required for optimal fertilization.