The polysialyltransferases interact with sequences in two domains of the neural cell adhesion molecule to allow its polysialylation

Abstract

Background: Recognition of the protein substrate is the first step in polysialylation of its glycans. Results: Residues in the OCAM Ig5 domain are non-permissive for its polysialylation. Conclusion: The polysialyltransferases interact with residues in both the Ig5 and FN1 domains of NCAM to allow its polysialylation. Significance: A two-domain polysialyltransferase recognition site may be required for all polysialylated proteins. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.