Research and design of quantitative interferometric microscopic cytometer

Abstract

Bio-ccll imaging is one of the important research directions in the biological and physical fields, especially the measurement and analysis of large amount of cells have major research value and significance in the bio-rcscarch and disease-diagnosis. However, the traditional microscopic detection is only restricted to the qualitative observation of cellular contours. Moreover, the amount of measurement samples is difficult to satisfy the statistical requirements due to the limitation of field of view. Thus a quantitative interferometric microscopic cytometer is designed based on the combination of quantitative interferometric microscopy and field of view scanning, and is used for the imaging and analysis of large amount of cells, in which the quantitative interferometric microscopy is capable of retrieving the cellular phase distribution, and of obtaining and analyzing a large amount of cellular information by combining the field of view scanning. The expanded principle component analysis algorithm, regularized optical flowing algorithm and Fourier phase retrieval algorithm are used, respectively. In addition, the different scanning modes are also combined, and thus the measurements and statistics of phase area, phase volume, circle ratio and other parameters of cells are obtained. The whole system is expected to obtain applications in high-throughput and high-speed cellular detection.