Virus-induced gene silencing (VIGS) is a widely used reverse genetics tool to knock down genes in plants transiently without transformation. The assay has been successfully used to downregulate the transcript abundance of a target gene at almost any plant developmental stages in any tissues. Here, we describe the VIGS assay using a barley stripe mosaic virus (BSMV) for functional genomics analysis in wheat with the focus on genes involved in rust resistance.
CITATION STYLE
Ali, S., & Hodson, D. (2017). Wheat Rust Diseases. In S. Periyannan (Ed.), Wheat Rust Diseases (Vol. 1659, pp. 257–264). Springer New York. Retrieved from http://link.springer.com/10.1007/978-1-4939-7249-4
Mendeley helps you to discover research relevant for your work.