Abstract
Targeted proteomics detects proteins of interest with high sensitivity, quantitative accuracy, and reproducibility. In a targeted proteomics assay, surrogate peptides are generated by proteolytic digestion of target proteins and selected reaction monitoring (SRM) assays are developed to quantify these peptides using liquid chromatography-tandem mass spectrometry (LC-MS/MS). In this report, we describe the details of quantitative analysis of target protein in cells and tissue samples.
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Chen, Y., & Liu, L. (2019). Targeted proteomics. In Methods in Molecular Biology (Vol. 1871, pp. 265–277). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8814-3_17
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