Comparação de métodos para a detecção de Fusarium graminearum em sementes de trigo (Triticum aestivum L.)

Abstract

The objective of this work was to compare different methods used in seed health testing for detection of Fusarium graminearum in wheat seeds. Twenty two treatments had been used: blotter test (BTDF) deep freezing; blotter test (BT); BT more 0.02% of 2,4-D; media half-selective (MSS); MSS more 0.02% of 2,4 - D ; MSS more KCl (-0,8 MPa); KCl, NaCl, manitol and sacarose in the osmotic potentials of -0,4, -0,2, -0,6 and -0,8 MPa. The used statistical delineation was entirely casualized, with four repetitions (two plates more substratum with 25 seeds each/repetition). There were no significant differences between of the different employed methods and the blotter test deep freezing, considered the method standard for seed health testing of grassy, for detection of the Fusarium graminearum in wheat seeds.