Syringin production by Saussurea medusa cell cultures in a novel bioreactor

Abstract

The culture of Saussurea medusa cell were cultured in an internal loop airlift bioreactor with sifter draft tube (ILABSDT) was investigated. Under the optimal culture conditions, which were inoculation size 1.5 g(d.m.) dm -3, aeration rate 0.3 dm3(air) dm-3(medium) min-1, and 14 mesh sifter holes, the maximum biomass, syringin content and syringin production reached 11.7 g(d.m.) dm-3, 17.7 mg g-1 and 206.6 mg dm-3, respectively. Among cell cultures in shake flask, bubble column bioreactor and ILABSDT, ILABSDT had the highest syringin productivity and reached 12.41 mg dm-3 d-1. © 2008 Institute of Experimental Botany, Academy of Sciences of the Czech Republic.