Cross-linking of chitosan membrane with polyethylene glycol diglycidyl ether for immobilization of uricase

Abstract

This paper describes the immobilization of uricase by entrapment. The entrapping membrane was prepared by the cross-linking of chitosan with polyethylene glycol diglycidyl ether (diepoxy compound). The properties of the immobilized enzyme were investigated and compared with those of the native uricase. The enzyme activity of the immobilized uricase were found to be more dependent on temperature and pH than that of the native enzyme. The uricase immobilized membrane was sufficiently stable for 30 days allowing for the determination of uric acid concentration. The uric acid sensor was constructed using a hydrogen peroxide electrode.