A simple and efficient method for derivatization of glyphosate and AMPA using 9-fluorenylmethyl chloroformate and spectrophotometric analysis

Abstract

This work presents the confirmation and optimization of the method for derivatization of glyphosate and aminomethylphosphonic acid (AMPA) using 9-fluorenylmethyl chloroformate (FMOCCl) evaluating the parameters: concentration of borate buffer and FMOC-Cl, homogenization time and of reaction, wavelength and solvent washing. The reaction was successfully promoted and the method is simple and easy to perform because it uses five minutes of homogenization and allows ultraviolet analysis immediately after the end of the reaction. © 2014 Sociedade Brasileira de Química.