Monooxygenase-catalyzed regioselective hydroxylation for the synthesis of hydroxyequols

Abstract

Monooxygenases exhibiting high activity and differing regioselectivity for the dietary isoflavone metabolite equol were discovered among enzymes in the HpaBC family by a genome mining approach. These enzymes enabled the one-step product-selective synthesis of 3′- and 6-hydroxyequols from equol and molecular oxygen.