Determination of Bioactive Compounds and Anti-cancer Effect from Extracts of Korean Cabbage and Cabbage

Abstract

In this study, we determined total polyphenol content(TPC) and total flavonoid content(TFC) of extracts from Korean cabbage and cabbage using a spectrophotometric method as well as glucosinolates concentration by HPLC. TPCs of Korean cabbage and cabbage extracts were 308.48 ㎍ GAE/g dry weight and 344.75 ㎍ GAE/g dry weight, respectively. TFCs of Korean cabbage and cabbage extracts were 5.33 ㎍ QE/g dry weight and 5.95 ㎍ QE/g dry weight, respectively. We found six different glucosinolates, namely progoitrin, glucoalyssin, gluconapin, glucobrassicanapin, glucobrassicin and 4-methoxyglucobrassicin in the Korean cabbage extract. In the cabbage extract, there was four glucosinolates, namely glucoraphanin, sinigrin, glucobrassicin and 4-methoxyglucobrassicin. We determined the cytotoxic effect of Korean cabbage and cabbage extracts in AGS human stomach cancer cells, HepG2 human hepatic cancer cells and LNCaP human prostate cancer cells by MTT assay. Dose-dependent relationships were found between the extract concentrations and cancer cell growth inhibition. The overall results support that both Korean cabbage and cabbage, the major vegetables in Korea, contain bioactive compounds such as polypheol, flavonoids as well as glucosinolates and they may play a positive role in cancer prevention. 서 론 십자화과 채소의 일종인 배추(Brassica campestris L. ssp. pekinensis)와 양배추(Brassica oleracea var. capitata)는 우리나 라, 중국, 일본 등지에서 널리 재배되고 있다(McNaughton & Marks 2003). 배추와 양배추에는 glucosinolates라는 독특한 생 리활성 물질이 있으며, 이들 성분의 섭취는 소화기계, 폐 등 의 암 발생을 억제한다고 보고되고 있으며(Fenwick 등 1983; van Poppel 등 1999), 최근 이들 채소의 섭취가 증가하고 있는 추세이다. 십자화과 채소에 널리 포함된 glucosinolates는 황(sulfur)을 함유하고 있으며, 휘발성이 강한 독특한 매운 향기를 부여한 다(Fenwick 등 1983). 현재까지 약 100여종의 glucosinolates가 확인되었으며, 그 중 30여종이 생리적인 활성을 갖고 있는 것 으로 알려져 있다(Fahey 등 2001). 손상되지 않은 채소에서 glucosinolate는 비활성인 상태로 존재하지만, 조리나 가공 중 에 채소를 자르고, 다지고, 으깨는 과정에서 채소 자체에 함유 된 myrosinase(thioglycoside glucohydrolase, EC 3.2.3.1)라는 효