High performance liquid chromatographic method development and its validation for lacidipine

Abstract

A simple and sensitive high performance liquid chromatographic (HPLC) method was developed for quantification of lacidipine in human plasma. Felodipine was used as an internal standard (IS). The present method used protein precipitating extraction of lacidipine from plasma. Separation was carried out on reversedphase c18 column (250 mm × 4.6 mm, 5μ) and the column effluent was monitored by UV detector at 320 nm. The mobile phase used was methanol: 0.5 mm ammonium acetate (ph 4.0), (70: 30 % v/v) at a flow rate of 1.0 mL/min. This method was linear over the range of 25.0 - 150.0 ng/mL with regression coefficient greater than 0.99. The method was found to be precise, accurate and specific during the study. The simplicity of the method allows for application in laboratories that lack sophisticated analytical instruments such as LC-MS/MS or GC- MS/MS that are complicated, costly and time consuming rather than a simple HPLC-UV method. The method was successfully applied for pharmacokinetic study of lacidipine.