Profiling of antioxidant enzymes in cat fish (Clarias batrachus) exposed to phenolic compounds

Abstract

Pollution of water sources due to chemicals plays a primary role in the destruction of ecosystems but chemical analyses alone may\rnot suffice to describe the adverse effects of the complex mixtures of chemicals present at contaminated sites. The potential utility\rof biomarkers for monitoring both environmental quality and the health of organisms inhabiting in the polluted ecosystems has\rreceived increasing attention during the last years. In the present investigation, the antioxidant profile of Clarias batrachus, a\rfresh water fish was determined by evaluation of antioxidant enzymes, SOD activity, catalase activity, glutathione peroxidase\ractivity, glutathione-S-transferase activity, reduced glutathione, level of conjugated dienes, hydrogen peroxidase activity and\rmalondialdehyde level. The studies showed that in fishes after exposure to phenolic compounds (treated group), there was a\rsignificant abnormal level of all the parameters as mentioned above at P<0.05 in comparison to normal (untreated group).