Material properties and characterizations of cross-linked electro-spinning raspberry ketone incorporated polyvinyl alcohol/gelatin fibrous scaffolds

Abstract

The properties of polyvinyl alcohol/gelatin (PVA/GE) nanofibers have been previously investigated as a function of the processing parameters such as the ra-tios of PVA and GE, electrical field and tip-to-collector distance during the electro-spinning process, in this study, the properties of the electro-spinning PVA/GE nanofibers were examined when different solution feed rates were used to create the fibrous scaffold. The optimal conditions for the PVA/GE fibrous scaf-fold were determined to be a PVA/GE blend ratio of 8/2, electrical field of 24 kV, tip-to-collector distance of 10 cm and speed rate of 1 ml.h-1. Using these con-ditions, Raspberry ketone (RK) was incorporated into PVA/GE fibrous scaffolds and their microstruc-ture and material properties were characterized by SEM, DSC and XRD techniques. When the incorpo-rated RK and PVA/GE fibrous scaffolds were cross-linked, the tensile strength and water-resistant ability increased at increasing cross-linking time. However, in the in vitro analysis, a longer cross-linking time was shown to increase its cytotoxicity. The cytotoxic-ity of RKPVA/GE-8 fibrous scaffold was evaluated based on a cell proliferation study by culturing L-929 fibroblast cell on the fibrous scaffold for 1, 3 and 5 days. In these experiments, cell expansion was ob-served and the cells spread during the entire cell cul-ture time.