Screening and optimization of pectin lyase and polygalacturonase activity from ginseng pathogen cylindrocarpon destructans

Abstract

Cylindrocarpon destructans isolated from ginseng field was found to produce pectinolytic enzymes. ATaguchi's orthogonal array experimental design was applied to optimize the preliminary production ofpolygalacturonase (PG) and pectin lyase (PL) using submerged culture condition. This method was appliedto evaluate the significant parameters for the production of enzymes. The process variables were pH, pectinconcentration, incubation time and temperature. Optimization of process parameters resulted in high levelsof enzyme (PG and PL) production after ten days of incubation at a pH of 5.0 at 25°C in the presence of1.5% pectin. Among different nitrogen sources, urea and peptone showed high production of PG and PL,respectively. The enzyme production and mycelial growth seems to have direct influence on the cultureconditions; therefore, at stationary state high enzyme production and mycelial growth were obtained thanagitation state. Along with this, optimization of enzyme activity was also determined using variousphysiological parameters like, temperature, incubation time and pH. Taguchi's data was also analyzed usingone step ANOVA statistical method.