The active compound of soursop leaf extract (Annona muricata, L.) as anti-vaginal discharge (Fluor albus)

Abstract

The aims of this study is to determine the effectiveness of antifungal Soursop leaf extract against the fungus Candida albicans and determine the class of compounds that act as vaginal discharge. Soursop leaf was extracted using maceration method with 96% ethanol solvent then concentrated with a rotary evaporator. The class determination of active compound by using multilevel composition fractionation method with n-hexane, chloroform, and ethyl acetate solvent. The effectiveness test of antifungal of each fraction toward Candida albicans by diffusion method is so that the researcher can look at the inhibitory zone diameter, then identify them by using UV-VIS and FTIR spectrophotometry to determine the active compound as antifungal. The highest antifungal effectiveness of soursop leaf active compounds was the n hexane fraction with inhibition diameter 23.7 mm which was categorized as strong, compared to the positive control of ketoconazole with inhibition zone 22.5 mm and nystatin 15, 9 mm. The compound which is thought to have antifungal activity from the n- hexane fraction of soursop leaves is a terpenoid compound.