Utilization of molasses wastewater for medium production of Bacillus cereus

Abstract

This study aims to analyze the potential of molasses wastewater as a production medium for the Bacillus cereus SN7 and B. cereus consortium. The research was conducted experimentally using several B. cereus strains such as B. cereus SN7, B. cereus SP4, B. cereus S5, B. cereus Xmb051, and B. cereus BF2. The Analysis of the potential of molasses wastewater as a production medium was carried out by growing the B. cereus SN7 and B. cereus consortium on molasses liquid waste medium with added liquid skim milk. In the comparison, the nutrient broth medium was used as a positive control, and sterile distilled water as a negative control. Bacterial growth measurements were carried out for 24 hours with different observations, sequentially, 0, 6, 12,18, and 24 hours and observed every six hours using the total plate count method. It can be shown that the highest cell biomass is 1,029 g/ml and the optimum growth time of each treatment is at 18th hours. Thus, the use of molasses as a bacterium medium can be used for the production of B. cereus SN7, and B. cereus consortium.