Novel compound for identifying Escherichia coli

Abstract

A new chromogenic compound, 5-bromo-4-chloro-3-indoxyl-β-D-glucuronide, was found to be useful for the rapid, specific, differential identification of Escherichia coli in the sanitary analysis of shellfish and wastewater. Of 1,025 presumptively positive colonies (blue) and 583 presumptively negative colonies (nonblue), only 1% false-negative and 5% false-positive results were found.