Sample Screening and Determination of 214 Veterinary Drug Residues in Food Using Chromatography–High-Resolution Mass Spectrometry

Abstract

Abstract: A combination of sample screening and subsequent confirmatory detection of contaminants represents an optimal strategy for routine analysis in implementing food safety monitoring programs. We propose ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry for the multicomponent determination of a wide range of veterinary drugs and their metabolite residues with minimal sample preparation. Isotopic dilution was employed to mitigate matrix effects. The procedure proposed in this study enables the simultaneous identification and assessment of residual levels of 214 veterinary drugs at limits of detection of 0.1–10.0 ng/g with a significantly shortened analysis time (40–50 min) and reduced cost.