A Rapid Colorimetric Method for the Determination of Iron in Biological Samples using o-Phenanthroline Method

Abstract

A simple, sensitive and highly selective direct colorimetric method for the determination of Iron in biological samples is developed. Iron reacts with o-phenanthroline and reduced from Fe3+ to Fe2+ by the use of an excess of hydroxylamine HCl to form a orange red complex ion. It was measured at an absorption maximum of 510 nm. Beer’s law was obeyed in the concentration range of 20-200 µg/mL. The method was applied successfully to a number of biological samples (female wistar rats’ liver), plant samples (pearl millet seeds). The method is very simple and requires no solvent extraction or pre-concentration steps. © 2018 iGlobal Research and Publishing Foundation. All rights reserved