Objective, comparative assessment of the penetration depth of temporal-focusing microscopy for imaging various organs

  • Rowlands C
  • Bruns O
  • Bawendi M
  • et al.
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Abstract

© The Authors. Temporal focusing is a technique for performing axially resolved widefield multiphoton microscopy with a large field of view. Despite significant advantages over conventional point-scanning multiphoton microscopy in terms of imaging speed, the need to collect the whole image simultaneously means that it is expected to achieve a lower penetration depth in common biological samples compared to point-scanning. We assess the penetration depth using a rigorous objective criterion based on the modulation transfer function, comparing it to point-scanning multiphoton microscopy. Measurements are performed in a variety of mouse organs in order to provide practical guidance as to the achievable penetration depth for both imaging techniques. It is found that two-photon scanning microscopy has approximately twice the penetration depth of temporal-focusing microscopy, and that penetration depth is organ-specific; the heart has the lowest penetration depth, followed by the liver, lungs, and kidneys, then the spleen, and finally white adipose tissue.

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APA

Rowlands, C. J., Bruns, O. T., Bawendi, M. G., & So, P. T. C. (2015). Objective, comparative assessment of the penetration depth of temporal-focusing microscopy for imaging various organs. Journal of Biomedical Optics, 20(6), 061107. https://doi.org/10.1117/1.jbo.20.6.061107

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