Antiurolithiatic activity of selected plants extracts against calcium oxalate crystals

Abstract

The present study was designed to evaluate the antiurolithiatic activity of selected plants extracts (Achyranthes aspera, Lawsonia inermis, Ficus benghalensis, Raphnus sativus and Macrotyloma uniflorum). The methanol extract of selected plants was analysed for in-vitro antiurolithiatic activity using nucleation, aggregation and growth assay of calcium oxalate (CaOX) monohydrate crystals. The nucleation, aggregation and growth of CaOx crystals were significantly inhibited by all selected plant extracts. The highest inhibition of CaOX nucleation was shown by R. sativus (55.21±1.9%) followed by M. uniflorum (53.91±1.1%) and the least by F. benghalensis (43.63±0.8%) at 1.0 mg/mL. The highest inhibition of calcium CaOX aggregation was shown by R. sativus (61.6±1.6%) which was very close to Cystone (63.28±2.5%). The growth of CaOX was highly inhibited by A. aspera (42.17±1.0%) followed by M. uniflorum (40.27±1.4%) with lowest inhibition by F. benghalensis (31.44±1.4%) as compared to Cystone (43.35 ±0.9%). The study showed that the selected plants showed the significant antiurolithiatic activity against CaOX crystals, which could be a potential source for the treatment of renal stone disease. Key words: Antiurolithiatic, calcium oxalate, nucleation, aggregation, methanol extracts.