Journal ArticleOPEN ACCESS

Genome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase

Wellcome Open Research (2023) 7
DOI: 10.12688/wellcomeopenres.18575.2
5Citations
Citations of this article
9Readers
Mendeley users who have this article in their library.
Get full text

Abstract

We present the genome sequence of Leishmania mexicana MNYC/BZ/62/M379 modified to express Cas9 and T7 RNA-polymerase, revealing high similarity to the reference genome (MHOM/GT2001/U1103). Through RNAseq-based annotation of coding sequences and untranslated regions, we provide primer sequences for construct and sgRNA template generation for CRISPR-assisted gene deletion and endogenous tagging.

Author supplied keywords

Cite

CITATION STYLE

APA

Beneke, T., Dobramysl, U., Catta-Preta, C. M. C., Mottram, J. C., Gluenz, E., & Wheeler, R. J. (2023). Genome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase. Wellcome Open Research, 7. https://doi.org/10.12688/wellcomeopenres.18575.2

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free