The potential of the isolated probiotics bacterial from giant prawns' digestive tract (Macrobrachium rosenbergii, de Man) with 16s Rdna Sequencing Technique

Abstract

Probiotics is a bacterial product that has the advantage for fish; it is not only for growing but also for covering the bacteria's attack. The aim of the study is to determine the types of candidate bacteria's probiotic of which the digestive tract of giant prawns in Teratak Buluh Pekanbaru, Riau. This research was conducted at the Laboratory of Microbiology and Biotechnology of Fisheries and Marine Sciences faculty, University of Riau, Pekanbaru to get a candidate of Probiotic Bacteria and conduct molecular examination that consists of purification of electrophoresis gel and 16S rDNA sequencing at the Institute for Biotechnology Serpong, Tangerang Banten. The method used in this researh was physical, biochemical and molecular test in order to identify the candidate bacteria. The results showed that the are eleven isolates of bacterial are the species Bacillus BDU4 strain from A Bacteria isolates with 99% homology and 10 isolates of bacterial are bacillus genus because the degree of homology below 97% and they are considered as the original indonesia's new species probiotic bacteria.