Efficiency of PowerCore in core set development using amplified fragment length polymorphic markers in mungbean

Abstract

The mungbean [Vigna radiata (L.) Wilczek] is a member of the Fabaceae, consists of around 20000 species. With the rapid increase in the number of germplasm collections, many gene banks face problems of redundant resources. To cope with this problem, the development of an allele-mining set is especially important. Our present study supports the efficiency of PowerCore in the development of core set from 705 collected accessions using AFLP markers. The result demonstrated the higher allele (fragment) capturing efficiency of PowerCore than other strategies (distance-based, stratified random and random) tested at any level of sample size (5%, 10%, 15%, 20% of total accessions) selected. Highly significant correlation (r=0.96) was observed between allele frequency distribution of entire collections and that of core set developed by PowerCore. The resulted core set was confirmed with 15 SSR data. The result will be useful, especially for reducing the redundant resources in the gene bank and allele mining from a large germplasm collection. © 2011 Blackwell Verlag GmbH.