Glut4 is sorted from a Rab10 GTPase-independent constitutive recycling pathway into a highly insulinresponsive Rab10 GTPase-dependent sequestration pathway after adipocyte differentiation

Abstract

Background: AS160 regulates insulin-sensitive glucose transport in adipocytes. Results: Knockdown of the AS160 substrate Rab10 differentially affected Glut4 and LRP1 in adipocytes and fibroblasts. Conclusion: Glut4 and LRP1 are sorted from a Rab10-independent constitutive trafficking pathway into a highly regulated Rab10-dependent pathway in adipocytes. Significance: Rab10 is a marker for the specialized insulin-sensitive pathway in adipocytes and an AS160 substrate that limits exocytosis through this pathway.