Seminal Fructose Concentration in Man Infertility and the Fructose Test's Meaning In Diagnosis Reason of Azoospermia Man

Abstract

Introduction WHO 2010 divided man infertility into 3 groups: normozoospermia (sperm concentration >=15 billion/ml), oligozoospermia (sperm concentration < 15 billion/ml) and azoospermia (no sperm) [1]. There are many studies determined seminal fructose and its correlations with concentration, vitality and motility of sperm. Azoospermia is the reason in 20% infertility man [2] and the common reason of azoospermia is CBAVD [3], [4]. The purpose of the studies is using seminal fructose test to replace percutaneous epididymis sperm aspiration (PESA) to diagnosis CBAVD. Fructose is essential for spermatozoa metabolism and spermatozoa motility [5]. Fructose is an energy source for spermatozoa. It is produced by the seminal vesicles with some contribution from the ampulla of the ductus deferens [6,7]. Determination of seminal fructose concentration has been used in examination of obstructive azoospermia and inflammation of male accessory glands [8,9]. The role of fructose concentrations in seminal plasma for total and sperm density has been investigated by several authors. Rajalakhshmi M et al. [10] and Gonzales GF [11] reported that an increase in sperm concentration is often accompanied by a decrease in fructose concentration in seminal plasma, because sperm using fructose as the primary source of energy [10,11]. However, others studies have also shown that fructose concentrations in seminal plasma of patients with oligozoospermia and azoospermia did not decrease as compared to normal men. Materials and Methods The studies select semen of infertility men (age 18-50) visited in Centre of genetics counselling of Hanoi medical university hospital from 3/2017-5/2018. After analyzing the semen, results of sperm concentration, vitality and motility were selected. Random choose 60 normozoospermia cases and 60 oligozoospermia cases to determine seminal fructose concentration by ROE improved method [12]. Fructose content in seminal plasma was determined Abstract Background: This is a review from 2 studies that determine the concentration of seminal fructose and show the correlations between seminal fructose concentration with sperm concentration, vitality and motility (progressive). Besides, these studies also show the reason of man infertility cases which azoospermia and no seminal fructose. Materials and methods: Include random 60 normozoospermia, 60 oligozoospermia semen. Determine fructose concentration by ROE method. 25 azoospermia cases with no fructose had been examined and proceed percutaneous epididymis sperm aspiration (PESA) to find the infertility reason.