Protein kinase C inactivation by Fenton's-reaction at discrete Cu++ binding sites

Abstract

The consequence of direct exposure to HO. radical (chemically generated by Fenton's reaction) of partially purified rat liver PKC has been evaluated in this work. PKC inhibited Fenton-dependent HO. generation, probably due to the binding of copper ions to the enzyme. PKC activity was inhibited by H2O2 Copper ions were able to increase the H2O2-mediated damage to the enzyme, but only beyond a concentration threshold. The possible interactions between PKC and Fenton's reagents, in particular copper ions, is discussed.