Deletion mutagenesis has been used to identify essential regions for rescue of coronavirus defective RNAs (D-RNAs). Using this technique on a cloned IBV D-RNA CD-91, we have identified a region potentially important in its rescue. Comparing the sequence of D-RNAs rescued with those not rescued we have deduced that a 72 base region corresponding to base number 13824 to 13896 in the viral genome is required for rescue. This may be an IBV D-RNA packaging signal or a cis-acting element involved in replication. Further experiments and modification of our techniques will be required to differentiate between the two processes.
CITATION STYLE
Dalton, K., Penzes, Z., Wroe, C., Stirrups, K., Evans, S., Shaw, K., … Cavanagh, D. (1998). Sequence elements involved in the rescue of IBV defective RNA CD-91. Advances in Experimental Medicine and Biology, 440, 253–257. https://doi.org/10.1007/978-1-4615-5331-1_32
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