Treatment of experimental periodontal disease by photodynamic therapy in immunosuppressed rats

Abstract

Fernandes LA, de Almeida JM, Theodoro LH, Bosco AF, Nagata MJH, Martins TM, Okamoto T, Garcia VG. Treatment of experimental periodontal disease by photodynamic therapy in immunosuppressed rats. J Clin Periodontol 2009; 36: 219-228. doi: 10.1111/j.1600-051X.2008.01355.x. Abstract Background and Objective: The aim of this study was to compare photodynamic therapy (PDT) as an adjunctive treatment of induced periodontitis with scaling and root planing (SRP) in dexamethasone-inhibited rats. Material and Methods: The animals were divided into two groups: ND (n=90), saline solution treatment; D (n=90), dexamethasone treatment. In the ND and D Groups, periodontal disease was ligature-induced at the first mandibular molar. After 7 days, the ligature was removed and all animals received SRP and were divided according to the following treatments: SRP, saline solution; Toluidine Blue-O (TBO), phenothiazinium dye; and PDT, TBO and laser irradiation. Ten animals in each treatment were killed at 7, 15 and 30 days. The radiographic and histometric values were statistically analysed. Results: In the ND and D Groups, radiographic analysis showed less bone loss in animals treated by PDT in all the experimental periods than SRP and TBO at 15 days (p<0.05). After a histometric analysis was carried out in the ND and D groups, the animals treated by PDT showed less bone loss in all periods than SRP and TBO after 15 days (p<0.05). Conclusions: The PDT was an effective adjunctive treatment of induced periodontitis compared with SRP in dexamethasone-inhibited rats. © 2009 John Wiley & Sons A/S.