Pre-anthesis reserve utilization for protein and carbohydrate synthesis in grains of wheat

Abstract

We assessed the contribution of pre-anthesis reserve C to protein and carbohydrate deposition in grains of wheat (Triticum aestivum L.) using a new approach comprised of steady-state 13C/12C labeling and separation of the protein and carbohydrate fractions of mature grains. Experiments were performed with two spring wheat cultivars (Kadett and Star) grown with differential N fertilizer supply over 2 years. Pre-anthesis reserves contributed between 30% and 47% of the C in protein and 8% to 27% of the C in carbohydrates of grains. Partitioning of pre-anthesis C among the grain fractions was strongly dependent on the C/N (w/w) ratio in mobilized preanthesis biomass (r2 = 0.92). There appeared to be no significant exchange of pre-anthesis C between amino acids and carbohydrates during redistribution. The mean apparent efficiency of mobilized carbohydrate-C use in grain filling (ME(CHO), estimated as the mass of pre-anthesis C deposited in grain carbohydrates per gram of preanthesis C mobilized from carbohydrates in vegetative plant parts) was 0.72, whereas that of protein-C (ME(p)) was 0.56. However, ME(p) and ME(CHO) varied among treatments. ME(CHO) increased with increasing contributions of water-soluble carbohydrates to total preanthesis carbohydrate mobilization. ME(p) decreased with increasing residence time of protein in vegetative biomass. Possible causes for variability of ME(p) and ME(CHO) are discussed.