Neuroprotective effect of butin against rotenone-induced Parkinson’s disease mediated by antioxidant and anti-inflammatory actions through paraoxonase-1-induction

Abstract

This investigation aimed to assess the anti-Parkinson's effect of butin in rotenone-treated rats. A total of 24 albino Wistar rats were segregated into four groups equally as follows: Group I (normal) and Group II (rotenone control); Group III and Group IV; were butin 25 and 50 mg/kg/p.o. Daily, 1 h post offer mentioned treatments, Group I animals were injected with sunflower oil (s.c.), and Group II-IV were treated subcutaneously with 2 mg/kg rotenone emulsion in sunflower oil for 28 days. On day 29, i.e. 24 h after the rotenone dose, animals were evaluated for behavioural and biochemical parameters i.e. acetylcholine-esterase, paraoxonase-1, butyrylcholinesterase, endogenous antioxidants (Glutathione, catalase, superoxide-dismutase), oxidative, nitrative stress and proinflammatory markers (Interleukin-6, IL-1β, and TNF-α). Butin attenuated the rotenone-induced catalepsy, akinesia, memory loss and decreased learning ability. Butin treatment shows rotenone-induced neurobehavioral impairments, oxidative and nitrative stress, and neuroinflammatory markers reversed the enzyme activities towards normal.