Inositol trisphosphate metabolism in Saccharomyces cerevisiae: Identification, purification and properties of inositol 1,4,5-trisphosphate 6-kinase

Abstract

Ins(1,4,5)P3 metabolism was examined in Saccharomyces cerevisiae extracts. S. cerevisiae contains readily detectable Ins(1,4,5)P3 kinase activity that is predominantly soluble, but phosphomonoesterase activity acting on Ins(1,4,5)P3 was not detected in either soluble or particulate preparations from this organism. We have purified the kinase activity ~ 685-fold in a rapid four-step process, and obtained a stable preparation. The enzyme has an apparent native molecular mass of ~ 40 kDa, and displays Michaelis-Menten kinetics with respect to its two substrates, ATP and Ins(1,4,5)P3. The K(m) for ATP was 2.1 mM, and that for Ins(1,4,5)P3 was 7.1 μM. The enzyme appeared to be the first step in the conversion of Ins(1,4,5)P3 into an InsP3, and the partially purified preparation contained another activity that converted the InsP4 product into an InsP3. The InsP4 product of the partially purified kinase was not metabolized by human erythrocyte ghosts and co-chromatographed with an Ins(3,4,5,6)P4 [L-Ins(1,4,5,6)P4] standard, identifying it as D-Ins(1,4,5,6)P4. The yeast enzyme is thus an Ins(1,4,5)P3 6-kinase. This activity may be an important step in the production of inositol polyphosphates such as InsP3 and InsP6 in S. cerevisiae.