Dietary supplementation of papaya (Carica papaya l.) leaf affects abundance of Butyrivibrio fibrisolvens and modulates biohydrogenation of C18 polyunsaturated fatty acids in the rumen of goats

Abstract

Saturated fatty acid (SFA) produced as a final product of rumen biohydrogenation (BH) by rumen microbes is harmful to human health because of increasing the risk of heart-related diseases. The objectives of this study were to test the effect of papaya leaf (PL) on the BH of C18 polyunsaturated fatty acids (PUFA), fatty acid composition and bacterial abundance in the rumen of goats. Three rumen fistulated male goats were assigned in a 3 × 3 latin square design using three levels of PL; no addition of PL in basal diet (control, CON, 50% concentrate +50% alfalfa hay), 25% of alfalfa hay in basal diet replaced by PL (medium PL, MPL) and 50% of alfalfa hay in basal diet replaced by PL (high PL, HPL). Supplementation of the diet with PL significantly (p