Study of grifola frondosa polysaccharide (GFP) on peritoneal macrophage activities of the long-term heavy load exercising mouse

Abstract

Long-term heavy-load exercise can decrease macrophage activity. In this study, we separated 50 Kunming (KM) mice into comparison groups to investigate the immune-regulatory effects of Grifola frondosa polysaccharide (GFP) on long-term heavy-load exercising mice. We evaluate NO content, macrophage survival and growth, intracellular GSH and macrophage phagocytosis 4 weeks after gavaging long-term heavy-load exercising mice with GFP. After exercise, NO content, macrophage survival and growth, phagocytic index significantly reduced in the mice not fed GFP. Both medium and high doses of GFP drastically increased NO, phagocytic index and decreased intracellular GSH. High doses of GFP also increased macrophage survival and growth. With this study, we demonstrate that four weeks of heavy-load exercise can weaken peritoneal macrophage activity. A supplement of GFP fed to these mice improved their peritoneal macrophage activity. The effect for the high-dose GFP treatment is especially significant.