A new method for measuring the size of nematodes using image processing

Abstract

Many studies have been made on nematodes, especially Caenorhabditis Elegans, which are used as a model organism. In many studies, the size of the nematode is important. This article describes a method of measuring the length, volume and surface area of nematodes from photographs. The method uses the imaging software ImageJ, which is in the public domain. Two macros are described. The first converts the images into binary form, and the second uses several built-in functions to measure the length of the worm and its diameter along its length. If it is assumed that the worm has a circular cross-section, then the volume and surface area of the nematode can be calculated. This is a cheap and easy technique.