Long-lived ionic nano-domains can modulate the stiffness of soft interfaces

Abstract

Metal ions underpin countless processes at bio-interfaces, including maintaining electroneutrality, modifying mechanical properties and driving bioenergetic activity. These processes are typically described by ions behaving as independently diffusing point charges. Here we show that Na + and K + ions instead spontaneously form correlated nanoscale networks that evolve over seconds at the interface with an anionic bilayer in solution. Combining single-ion level atomic force microscopy and molecular dynamic simulations we investigate the configuration and dynamics of Na + , K + , and Rb + at the lipid surface. We identify two distinct ionic states: the well-known direct electrostatic interaction with lipid headgroups and a water-mediated interaction that can drive the formation of remarkably long-lived ionic networks which evolve over many seconds. We show that this second state induces ionic network formation via correlative ion-ion interactions that generate an effective energy well of -0.4k B T/ion. These networks locally reduce the stiffness of the membrane, providing a spontaneous mechanism for tuning its mechanical properties with nanoscale precision. The ubiquity of water-mediated interactions suggest that our results have far-reaching implications for controlling the properties of soft interfaces.