Development of a closed-artery catheter-based myocardial infarction in pigs using sponge and lidocaine hydrochloride infusion to prevent irreversible ventricular fibrillation

Abstract

The objectives of this study were to develop a robust, homogeneous, viable and inexpensive model of closed-artery catheter-based model of myocardial infarction (MI) in pigs without major cardiac dysfunction. Suitable animal models that mimic human cardiovascular conditions are of paramount importance to understand the effects of novel therapeutic strategies to improve tissue perfusion and prevent cardiac deterioration post-MI. Pigs (N = 21, BW = 17 ± 1 kg) receiving continuous iv lidocaine hydrochloride were subjected to percutaneous intracoronary implant of foam sponge into the proximal left circumflex coronary artery. Intraprocedure mortality was 23.8%. ST segment elevation and increased serum Troponin T and CK-MB were documented in all animals. Thirty days after occlusion, echocardiography (95% IC [9.3-12.4%]) and anatomopathological (95% CI [9.3-12.6%]) analyses confirmed a significant and reproducible MI. Taken together, we provide evidence for a suitable closed-artery catheter-based method to produce MI in pigs accompanied by tissue hypoperfusion and absence of overt heart failure.