Separation of megakaryocytes from mouse bone marrow by velocity sedimentation

Abstract

A technique for separating mouse bone marrow megakaryocytes by unit gravity velocity sedimentation is described using a modified sedimentation chamber. The velocity sedimentation profile for megakaryocytes comprises 3 peaks corresponding to sedimentation velocities of 30, 60, and 100 mm/hr. Morphologic examination of megakaryocytes in the 30 mm/hr peak reveals them to be primarily immature with the latter 2 peaks corresponding to larger, more mature megakaryocytes.