Phytochemical investigation and antifungal activity of Daucus littoralis Smith sub sp. hyrcanicus Rech.f

Abstract

Daucus littoralis Smith subsp. hyrcanicus Rech.f. (Caspian carrot) is an endemic species in the north of Iran. The aim of this study was to report the separation and structural elucidation of constituents from the ethyl acetate extract of fruits, which have not been reported previously. A phenylpropanoid (myristicin), a sesquiterpenoid (acorenone B) and two fatty acids (palmitic and oleic acid) were identified by GC and GC/MS. Also acorenone B and triolein were isolated by different chromatographic methods and identified by 1 H, 13 C-NMR and MS spectra. Furthermore, the antifungal activity of the Fruits Ethyl Acetate (FEE) and Fruits Methanol Extracts (FME) were investigated against vulvovaginal isolates of Candida albicans. The results showed that both FEE and FME had antifungal activity for standard Candida (MIC 6.25 and 25 mg mL -1, respectively) as well as for the isolates with MIC ranged 25-100 mg mL -1.