Identification of Three Major Components in Fish Sarcoplasmic Proteins

Abstract

Attempts were made to identify the native proteins (“43K”,“40K” and “35K” components) whose subunits appeared as three bands in SDS-gel electrophoretograms of sarcoplasmic proteins from each of red sea bream, Pacific mackerel and carp ordinary muscle. Judging from gel filtration behaviors on Sephadex G-150, along with subunit molecular weights and other properties, “43K”, “40K” and “35K” components were identified as creatine kinase, aldolase and glyceraldehyde-3-phosphate dehydrogenase, respectively. Specific activity of creatine kinase ranged from 25.9-33.3 units/mg through the three fish species, whereas those of aldolase and glyceraldehyde-3-phosphate dehydrogenase were more species-specific, ranging from 12.4-32.3 and 28.0-39.4 units/mg, respectively. Molecular weights of isolated creatine kinase, aldolase and glyceraldehyde-3-phosphate dehydrogenase were determined to be 86,000-88,000, 160,000 and 136,000-140,000, respectively, regardless of fish species. © 1988, The Japanese Society of Fisheries Science. All rights reserved.