STUDIES ON YEAST DIFFERENTIATION USING ORGANIC ACID METABOLITES PART 2. STUDIES ON THE ORGANIC ACID METABOLITES PRODUCED BY YEASTS GROWN ON GLUCOSE

Abstract

A survey using four strains of yeast was made to establish whether the organic acid metabolites produced by growth on synthetic medium using glucose substrate could provide a means of differentiation. While concentrations of the various acids produced were different, they were insufficiently unique to allow predictability of the strain from which they arose. High performance liquid chromatography on the resin Aminex HPX‐87H provided an ideal monitoring base but a number of components could not be identified from retention times of commonly occurring organic acid metabolites. Chromatograms and charts showing changes occurring throughout the fermentation are included. 1991 The Institute of Brewing & Distilling